2016
Volume 16, Number 4, pp. 172–176
Novel technology for the fast production of complex nanoliposomes
T. Mdzinarashvili,1, 2 M. Khvedelidze,2 E. Shekiladze1, 2 and R. Machaidze2
1Faculty of Exact and Natural Sciences, I. Javakhishvili Tbilisi State University, Georgia
2Institute of Medical and Applied Biophysics, I. Javakhishvili Tbilisi State University, Georgia
Incorporation of different chemical substances, including medicines, into liposomes has been increasingly evaluated in recent years due to their targeted drug delivery potential. Current technologies of nanoparticle-incorporating liposome preparation are relatively complex. We present a novel method of incorporating complex hydrophobic and hydrophilic molecules as well as simpler substances into dipalmitoylphosphatidylcholine (DPPC) or dipalmitoyl-sn-glycero-3-phosphatidic acid (DPPA) liposomes. In order to prepare these complex liposomes, for the first stage it is necessary for ligands and lipids to directly interact, followed by the addition of pH-buffered water or solvent at temperatures slightly above the liposome phase transition temperature. The resulting mixture is briefly but vigorously shaken and then transformed into liposomes of the desired size using an extruder. Particle sizing and calorimetry were used to evaluate liposome formation. This technology is simple, fast and economical, requiring no more than 30 min to prepare the complex liposomes—faster (and less expensive) than current methods. This technology can be usefully added to the armamentarium available to pharmacologists who desire to prepare complex liposomes.
Keywords: calcium, gold nanoparticles, liposome preparation, novel nanotechnology