2016
Volume 16, Number 4, pp. 167–171
Some criteria for the evaluation of endogenous intoxication in men with prostate tumours
L. Ramishvili,1 M. Zibzibadze,1 M. Alibegashvili,1 T. Chigogidze,2 M. Gordeziani,1 N. Gabunia,2 A. Khazaradze1 and N. Kotrikadze1
1Division of Cellular and Molecular Biology, Department of Biology, Faculty of Exact and Natural Sciences, Iv. Javakhishvili Tbilisi State University (TSU), 13 University St, 0186 Tbilisi, Georgia
2Department of Urology, Faculty of Medicine, Iv. Javakhishvili Tbilisi State University (TSU), 2 Chiaureli St, 0159 Tbilisi, Georgia
Functional parameters of the erythrocyte membrane have been studied along with a number of other biochemical parameters to assess the degree of endogenous intoxication in the blood of men with prostate tumours. The erythrocytes of the patients with benign prostatic hyperplasia (BHP), benign prostatic hyperplasia with high-grade prostatic intraepithelial neoplasia (BHP with HGPIN) and cancer of the prostate (CaP) served as material. The erythrocytes’ functional parameters and other biochemical parameters were determined spectrophotometrically: sorption capacity of erythrocytes stayed the same in the case of BHP and was increased in patients with BHP with HGPIN and CaP. The permeability of erythrocytes was only slightly increased in the case of prostate cancer. Primary and secondary products of lipid peroxidation decreased while the amount of final products sharply increased in all the studied groups. The amount of middle molecular weight substances (MMS) was raised in all patients and especially in the case of BHP with HGPIN. Increased sorption capacity of erythrocytes and accumulation of MMS confirm that the given criteria are informative and reliable for evaluation of endogenous intoxication. According to our results, permeability of the erythrocyte membrane does not represent an informative criterion for evaluation of endogenous intoxication in the case of prostate tumours. Endogenous intoxication was especially prominent in patients with BHP with HGPIN, indicating the aggressiveness and poor prognosis of this form of the pathology.
Keywords: endogenous intoxication, erythrocytes, lipid peroxidation, prostate tumours