2014
Volume 14, Number 3, pp. 54–56
Properties of ion channels formed by peroxiredoxin-6 in the lipid bilayers: cluster channel inactivation
P.A. Grigoriev, M.G. Sharapov and V.I. Novoselov
Institute of Cell Biophysics, Russian Academy of Sciencies, 142290 Pushchino, Moscow Region, Russia
We show that the antioxidant protein peroxirdoxin-6 forms cation-selective ion cluster-type channels in phosphatidylcholine bilayer membranes. The cluster channel consists of several (from three to ten) conducting subunits—monochannels—each with a conductance of about 350 pS in 200 mM KCl. Mean dwell time in the open state of the cluster is voltage-dependent. It exponentially diminishes with increasing membrane voltage. A possible molecular mechanism of the channel’s inactivation is discussed.
Keywords: ion cluster-channel, ion monochannel, potential-dependent inactivation