2012
Volume 12, Number 3, pp. 117–119
Influence of pretreatment of cellulose substrate on the quality of hydrolysis of plant biomass
I. Dzalamidze,1 N. Zakariashvili,1 L. Kutateladze,1 T. Urushadze,1 R. Khvedelidze,1 T. Aleksidze,1 M. Jobava1 and T. Torok2
1 Durmishidze Institute of Biochemistry and Biotechnology, Agrarian University of Georgia, Tbilisi
2 Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, MS 70A 3317, Berkeley, 94720 California, USA
An ecologically safe physical method, a combination of freezing and mechanical grinding, has been used to improve the quality of hydrolysis of plant biomass. Wheat stalks were selected among different plant substrates (wheat stalks, tomato and apple skins, orange peel) for testing the efficiency of the method. The chemical composition of intact and preliminarily processed stalk has been investigated. The positive effect of freezing and mechanical grinding on the quality of stalk enzymatic hydrolysis by A. versicolor D-1 was revealed. The optimal duration of hydrolysis of the treated stalks was established as 6 h.
Keywords: enzymatic hydrolysis, ethanol, wheat stalk