The Journal of Biological Physics and Chemistry

2020

 

Volume 20, Number 2, pp. 86-88

 

 

 

Ozone sterilization system for processing of small-sized dental instruments

V. Taran,1 I. Garkusha,1 V. Krasnyj,1 A. Lozina,1 A. Taran,1 V. Starikov,2 S. Starikova3 and A.G. Mamalis4

1 Institute of Plasma Physics of NSC KIPT, Kharkov, Ukraine

2 National Technical University "Kharkov Polytechnic Institute", Kharkov, Ukraine

3 Kharkov Medical Academy of Postgraduate Education, Kharkov, Ukraine

4 Project Centre for Nanotechnology and Advanced Engineering, NCSR "Demokritos", Athens, Greece

A portable device for cleaning and ozone sterilization of small, delicate dental instruments that cannot withstand the high heat and humidity of standard autoclaving has been developed. The device comprises an ultrasonic bath for precleaning and a container for ozone sterilization with a reactor based on dielectric barrier discharge. The maximum ozone concentration in water reached 8.5 mg/L after 10 minutes of operation at a water temperature of 19 °C. Results of inactivation of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus are presented. Distilled water seeded with the microorganisms was bubbled with an ozone-air mixture at an ozone concentration of 5.8 mg/L at 17 °C for 5, 10, 20 and 30 min. The greatest bactericidal effect was manifested for P. aeruginosa cells, which were completely eliminated within 10 min. Inactivation of E. coli required about 20 min, and S. aureus about 30 min.

Keywords: bacteria, cleaning, dental instrument, dielectric barrier discharge, sterilization

 

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