2010
Volume 10, Number 2, p.p. 55–62
Fluorescence study of interactions between a europium coördination complex and model membranes
Andrey Yudintsev,1, * Valeriya Trusova,1 Galyna Gorbenko,1 Todor Deligeorgiev,2 Aleksey Vasilev,2 Stefka Kaloianova2 and Nedjalko Lesev2
1 Department of Biological and Medical Physics, V. N. Karazin Kharkiv National University, 4 Svobody Sq., 61077 Kharkiv, Ukraine
2 Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia, 1164 Sofia, Bulgaria
Membrane partitioning and bilayer-modifying properties of a Eu(III) coördination complex (drug) were evaluated using equilibrium dialysis and absorption and fluorescence spectroscopies. To gain insight into the drug’s influence on the physical parameters and molecular organization of the lipid bilayer, several fluorescent probes have been employed: pyrene, 1,6-diphenyl-1,3,5-hexatriene (DPH), 4-p-(dimethylaminostyryl)-1-dodecylpyridinium (DSP-12) and 6-propionyl-2-(dimethylamino)naphthalene (Prodan). The high lipophilicity of the Eu(III) coördination complex together with its relatively weak membrane-modifying propensities are prerequisites for the development of liposomal formulations of this compound.
Keywords: bilayer modification, drug-lipid interactions, Eu(III) coördination complex, membrane partitioning