2014
Volume 14, Number 1/2, pp. 6–10
Study of CeO2 nanoparticle interactions with biological cells and lipid bilayers
A.L. Popov,1 I.I. Selezneva,1 N.S. Kurnakov,2 V.K. Ivanov2 and P.A. Grigoriev3
1 Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
2 Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Russia
3 Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
The action of different doses of CeO2 nanoparticles on HEp-2 cells, NCTC L929 fibroblasts and model lipid bilayer membranes has been studied. The MTT test of the mitochondrial dehydrogenase activity of the NCTC L929 fibroblasts and HEp-2 epithelial cells showed that there is no difference in the viability of the cells in media containing 10–5–10–11 M of CeO2, stabilized by sodium citrate, and the viability of the cells in medium without CeO2. It was shown that neither the lipid membrane conductance nor the surface charge of the lipid bilayer interface were changed in the 10–5–10–11 M range of nanoparticle concentrations. The results indicate low cell toxicity of the CeO2 nanoparticles.