Volume 2, Number 1/2, p. 47-52
T. Berishvili, M. Garuchava, Ch. Kakhniashvili and D. Pataraya
Durmishidze Institute of Biochemistry and Biotechnology, Georgian Academy of Sciences, David Aghmashenebeli Ave. 10th km, 380059 Tbilisi, Georgia.
Physico-chemical characterization and identification of 2,4-dichlorophenoxyacetic acid transformation products in soil microorganisms.
Physico-chemical indices of the main products of 2,4-dichlorophenoxyacetic acid (2,4-D) biotransformation in actinomycetes have been established and the herbicide transformation products have been identified. It was found that 2,4-D-degrading soil microorganisms perform decarboxy-demethylation of the herbicide. 2,4-D was identified as an intermediate product of 2,4-D side chain degradation. Simultaneously with this degradation ring hydroxylation occurs: 4-OH-2,3- and 4-OH-2,5-dichlorophenoxyacetic acids have been identified. A significant part of the herbicide undergoes complete benzene ring degradation and its carbon atoms are incorporated into low-molecular weight cell metabolites.
Keywords: actinomycetes, biotransformation, detoxification, herbicide, 2,4-D