2020
Volume 20, Number 1, pp. 32-36
Antioxidant activity of green tea catechins delivered in liposomes to Jurkat and MDCK cells
L. Lursmanashvili,1 E. Shekiladze,2,3 T. Mdzinarashvili,3 I. Chkhikvishvili,2 N. Kipiani,2 M. Enukidze,2 M. Machavariani,2 M. Khvedelidze,3 E. Lomadze2,3 and T. Sanikidze2
1 Faculty of Medicine, Davit Aghmashenebeli University, Tbilisi, Georgia
2 Department of Physics, Biophysics, Biomechanics and Informational Technologies, Tbilisi State Medical University, Tbilisi, Georgia
3 Faculty of Exact and Natural Sciences, I. Javakhishvili State University, Tbilisi, Georgia
The identification of natural compounds with effective pharmacological properties and the investigation of the molecular mechanisms of their activities are essential for the creation of pharmaceutical preparations for a wide range of common diseases. A useful increase of effectiveness and stabilization of phenolic compounds characterized by low solubility in water, poor absorption and fast metabolism can be achieved by means of liposomes. In this work the impact of 1,2-dipalmitoyl phosphatidic acid (DPPA) and dipalmitoyl phosphatidylcholine (DPPC) liposomes on the antioxidant activity of green tea catechins (GTC) using the experimental models of Jurkat and Madin-Darby canine kidney (MDCK) cells was examined. In order to model oxidative stress, 30% H2O2 was added to the cells' incubation medium. Cell viability was assayed by the MTT test. Results revealed selective sensitivity of Jurkat and MDCK cells to GTCs, which is related to the synergistic effect of the exogenous and endogenous antioxidant systems of the cells. The positively charged DPPC liposomes interact with the negatively charged cell membranes and strengthen the antioxidant effect of GTCs in both types of cells. Negatively charged DPPA liposomes weaken the effect of GTCs on MDCK cells incubated under intensive oxidative stress. We conclude that liposomes modulate bioactivity and therapeutic effects of GTCs.
Keywords: antioxidant activity, green tea catechins, liposomes, oxidative stress