2015
Volume 15, Number 1, pp. 5–8
Ethanol intoxication and remediation with antioxidants in vitro
D.P. Museridze, I.K. Svanidze, L.G. Gegenava and N.N. Gvinadze
Department of Neurotoxicology, I. Beritashvili Centre of Experimental Biomedicine, Tbilisi, Georgia
The effect of ethanol on axon growth and the migration of glial cells in organotypic cultures of nerve and muscle tissue and the mitigation of destructive changes with the antioxidants Dolivin and zinc sulfate were studied. Studies were conducted in cultures of cortical and hippocampal explants from 1–2-day old newborn rat brains, and spinal and somatic muscle explants from 14-day old chick embryos, within 24, 48 and 72 hours. Explants were cultured on a collagen substrate in a Maximov chamber bathed in Dulbecco’s modified Eagle’s medium and bovine serum. In intact cultures, the active migration of glial cells and directional growth of neuronal axons in the growth zone of explants were revealed. After the addition of ethanol to the nutrient medium, a significant reduction in the number of glial cells and inhibition of axon growth in the growth zone of explants was observed. Adding Dolivin and zinc sulfate to the medium containing ethanol caused stimulation of axonal growth and glial cell migration in the growth zone of explants, resulting in the formation of a network similar to neuropil in vivo.
Keywords:alcohol, Dolivin, growth zone, organotypic culture, zinc sulfate