The Journal of Biological Physics and Chemistry

The application of fullerene C60 for the modification of an anticancer vaccine based on metabolism products of

Bacillus subtilis 7025

G.V. Didenko,1 L.V. Sorokina,1 Eu.G. Shpak,1 O.A. Golub,2 O.G. Lysenko,3 G.P. Potebnya1 and

A.G. Mamalis4,*

1  R.E.Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences, Vasylkivska St 45, 03022 Kiev, Ukraine

2  Inorganic Chemistry Department, Taras Shevchenko National University, Kiev, Ukraine

3  V. Bakul Institute of Superhard Materials, National Academy of Sciences, Kiev, Ukraine

4  Project Centre for Nanotechnology and Advanced Engineering, National Centre of Scientific Research “Demokritos”,

   15310 Agia Paraskevi, Athens, Greece

The evaluation of the antitumour effectiveness of an anticancer vaccine (AV) based on sarcoma 37 tumour cells and Bacillus subtilis 7025 glycoprotein complexed with the fullerene C60 physically sorbed or chemically attached to aerosil was the aim of this work. It was established that the use of fullerene C60 for AV modification leads to the improvement of the antitumour properties of the vaccine.  Immunological investigations evidenced the stimulation of lymphocytes and the cytotoxic activity of peritoneal macrophages and an increase in the level of antibodies to sarcoma 37 antigen production. Hence, it may be concluded the modification of cancer vaccines with this nanomaterial significantly increases their antitumour effectiveness.