Nitrobenzene oxidizing enzymes in plant cells
T. Varazashvili, G. Khatisashvili, M. Kurashvili, M. Pruidze, T. Ananiashvili,
G. Zaalishvili and M. Gordeziani
Durmishidze Institute of Biochemistry and Biotechnology of the Academy of Sciences of Georgia,
David Agmasheneblis Kheivani 10 km, 350059 Tbilisi, Georgia
Nitrobenzene NADPH-, H2O2- and O2-dependent oxidation in soybean and maize subcellular fractions has been studied. It is demonstrated that in the presence of nicotinamide coenzyme, transformation via monooxygenase has decisive significance. H2O2- and O2-dependent transformation of nitrobenzene is accomplished via either enzymatic or non-enzymatic (radical) mechanisms which results in formation of m- and p-nitrophenols.
Keywords:
cytochrome P450, detoxification, monooxygenase, peroxidase,phenoloxidase, xenobiotic