2013
Volume 13, Number 4, pp. 125–130
Analysis of a curve fitting model in the amide region applied to the muscle tissues of an edible fish: Labeo rohita fingerlings
S. Karthikeyan1 and KR. Easwaran2
1 Department of Physics, Valliamai Engineering College, 603203 Kattankulathur, Tamil Nadu, India
2 Department of Physics, Dhaanish Ahmed College of Engineering, 601301 Chennai, Tamil Nadu, India
Fourier transform infrared spectroscopy (FTIR) is important in determining the secondary structure of proteins. Among the amide I and amide III spectral bands have been found the most sensitive variations of the secondary structure of proteins. This paper describes the steps undertaken to find and separate the hidden and overlapped peaks in the amide I region of the muscle tissues of an edible fish. The peaks were found by analysis of second derivative spectra and peak separation was carried out by curve fitting with four different models. The model that fit best was that of a pure Lorentzian peak. Shoulder limit and detection limit maps were used to validate the revealed spectral features.
Keywords: curve fitting, fish, FTIR, protein, second derivative