Volume 6, Number 4, p.p. 167-170
The analysis of wrong G-C pairs induced by the ions of transition metals and H3O+ in DNA
Vasil Bregadze* and Ketevan Tsakadze
E.Andronikashvili Institute of Physics, Georgian Academy of Sciences, 6 Tamarashvili St, 0177 Tbilisi, Georgia
Ultraviolet difference spectra (UDS) and atomic emission spectroscopy (AES) were combined in order to find the number of wrong Watson-Crick base pairs in DNA. Thes approach is based on the UV spectroscopic display of self-coordinated double proton transfer in the G-C pairs of DNA. AES was used for quantitative analysis of the trace elements. The combined use of the methods enabled an estimate of the number of wrong Watson-Crick base pairs in DNA, which determines the risk factor for different diseases and provides a measure of the quality of DNA for applications in nanotechnology. We have estimated the number of wrong Watson-Crick base pairs induced by H3O+, Mg2+, Ca2+, Cr3+, Fe3+, Co2+, Ni2+,, Cu2+, Zn2+, Cd2+ and Pb2+ ions.
Keywords:
AES, UDS, wrong Watson-Crick base pairs.