Volume 7, Number 3, p.p. 103–106
The binding strength of Cd(II) to C-phycocyanin from Spirulina platensis
Eteri Gelagutashvili,* Ana Khizanishvili,* Eteri Ginturi, Nana Kuchava, Nanuli Bagdavadze, Alexander Rcheulishvili and Liguri Mosulishvili
E. Andronikashvili Institute of Physics, 6 Tamarashvili Street, 0177 Tbilisi, Georgia
The binding energy of Cd(II) ions with the globular protein C-phycocyanin extracted from Spirulina platensis was determined using equilibrium dialysis and atomic absorption spectroscopy. Cooperative binding of cadmium ions with C-phycocyanin was observed. The binding constants diminished with increasing ionic strength of NaCl, suggesting an adaptive protective response.
Keywords: binding constant, Cd(II) ions, C-phycocyanin, equilibrium dialysis, Spirulina platensis