Volume 9, Number 2, p.p. 61–64
Dynamic trend of energy exchange intensity in the brain under chronic stress
M. Chipasvili,1 N. Zaalishvili,2 E. Juravleva,2 N. Koshoridze1 and D. Mikeladze2
1
Department of Biology, Iv. Javakhishvili State University, 2 University St, 0128 Tbilisi, Georgia
2
Department of Biochemistry, Iv. Beritashvili Institute of Physiology, 14 Gotua St, 0160 Tbilisi, Georgia
We studied the dynamic trend of changes in the activity of creatine kinase, aldolase and succinate dehydrogenase in brain cells under 30-day long stress induced by isolation and a violated diurnal cycle. It was shown that these enzymes responded heterogeneously to 30-day long stress. Particular sensitivity was noted for succinate dehydrogenase, which showed a decline of activity in various sections of the brain: some 60–80% on average. Unlike succinate dehydrogenase, aldolase activity increased on the 10th day of stress and then declined. Similar results were seen for phosphokinase activity. It was observed that the changes in the activities of the enzymes were accompanied by quantitative changes in nitric oxide (NO) concentration. We inferred from the experimental data that the main signalling molecule causing changes in enzyme activity is likely to be NO.
Keywords:
aldolase, brain, creatine kinase, stress, succinate dehydrogenase