Volume 6, Number 1, p.p. 15-18
Assessment of the functional state of immunocompetent cells enriched with 3,5-cholestadiene
N. Kobakhidze, N. Migineishvili, N. Sultanishvili, M. Mardaleishvili, D. Gamrekeli and M. Shengelia
Iv. Javakhishvili Tbilisi State University, Faculty of Biology, Laboratory of Molecular Mechanisms of Carcinogenesis, Department of Biophysics, 2 University St, 0186 Tbilisi, Georgia
The effects of a primary oxidative product of cholesterol, 3,5-cholestadiene, on rat spleen lymphocyte membrane microviscosity, permeability to Na,+ and K+ (Na+-K+-ATPase activity and changes of the total flux of ions) as well as on the activity of membrane-bound lipiddependent enzymes (5'-nucleotidase, glucose-6-phosphate and acid phosphatase) have been investigated. The incorporation of increasing concentrations of the oxidized steroid into the membranes led to an increase in membrane microviscosity and in the active transport of ions. It has been shown that the impact of the steroid on enzymes having different features and different localizations is similar.
Keywords: fluidity, lymphocyte, membrane-bound enzymes, permeability, 3,5-cholestadiene